CD302 Monoclonal antibody

CD302 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2B1E8

Cat no : 66640-1-Ig

Synonyms

BIMLEC, C type lectin BIMLEC, CD302, CD302 antigen, CD302 molecule, CLEC13A, DCL 1, DCL1, KIAA0022

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Validation Data Gallery

• 

  WB analysis of THP-1 using 66640-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66640-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DC2.4 using 66640-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 66640-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: THP-1 cells, DC2.4 cells, HL-60 cells, LPS treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66640-1-Ig targets CD302 in WB,ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD302 fusion protein Ag9873
• Full Name: CD302 molecule
• Calculated Molecular Weight: 170aa,19 kDa; 232aa,26 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC020646
• Gene Symbol: CD302
• Gene ID (NCBI): 9936
• RRID: AB_2881999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.