CoraLite® Plus 647 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF,FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF,FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

390

Cat no : CL647-65058

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Validation Data Gallery

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  IF Staining of bEnd.3 using CL647-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 647 CD31 antibody (CL647-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65058

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5.00 ul/test CoraLite® Plus 647-conjugated Anti-Mouse CD31 (CL647-65058, Clone: 390) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

• Positive IF detected in: bEnd.3 cells
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65058 targets CD31 in IF,FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: Pecam1
• Gene ID (NCBI): 18613
• RRID: AB_2883659
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).