FITC Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a

Reactivity

Mouse and More (2)

Applications

IF, FC

Conjugate

FITC Fluorescent Dye

CloneNo.

390

Cat no : FITC-65058

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1



Tested Applications

Positive IF detected inbEnd.3 cells
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65058 targets CD31 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse, rat
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D
Full Name platelet/endothelial cell adhesion molecule 1
GenBank accession numberBC008519
Gene symbol Pecam1
Gene ID (NCBI) 18613
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for FITC CD31 antibody FITC-65058Download protocol
FC protocol for FITC CD31 antibody FITC-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease.

Authors - Hong Su
mouseFC

Bone

mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification

Authors - Jiaming Fu
ratFC

Cells

Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage Polarization

Authors - Xufeng Mao