CoraLite® Plus 488 Anti-Mouse CD31 (390)

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  IF Staining of bEnd.3 using CL488-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 488 CD31 antibody (CL488-65058, Clone: 390 ) at dilution of 1:100.

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  FC experiment of mouse bone marrow cells using CL488-65058

  1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD31 (390) (CL488-65058, Clone: 390) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

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  FC experiment of mouse bone marrow cells using CL488-65058

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD31 (390) (CL488-65058, Clone:390) (red) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3) (blue). Cells were not fixed.

CD31 Monoclonal Antibody for IF/ICC, FC

Cat No. CL488-65058

Clone No.390

Reviews (1)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Synonyms

390, Pecam, PECAM 1, Pecam1, PECAM-1

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Tested Applications

• Positive IF/ICC detected in: bEnd.3 cells
• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  C3H/HeJ mouse hematopoietic progenitor cell line 32D

• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• RRID: AB_2883154
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q08481
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vanessa (Verified Customer) (09-27-2023)

Label but not very bright. Hard to distinguish from background.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain