Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65058 targets CD31 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
C3H/HeJ mouse hematopoietic progenitor cell line 32D Predict reactive species |
| Full Name | platelet/endothelial cell adhesion molecule 1 |
| GenBank Accession Number | BC008519 |
| Gene Symbol | CD31 |
| Gene ID (NCBI) | 18613 |
| RRID | AB_2883154 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q08481 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD31 antibody CL488-65058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for CL488-65058 rates it 3 out of 5. Used at a 1:100 dilution on brain tissue for immunohistochemistry and immunofluorescence, the reviewer found the antibody labeled the target but the signal was not very bright and was difficult to distinguish from background. Overall, the feedback suggests the product works but may require optimization to improve signal-to-background ratio.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vanessa (Verified Customer) (09-27-2023) | Label but not very bright. Hard to distinguish from background.
|
