APC Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF/ICC, FC
Cat No. APC-65058
Clone No.390

Host / Isotype

Rat / IgG2a

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC

C85791, Cd31, Pecam, PECAM 1, Pecam1

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inbEnd.3 cells
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

APC-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D Predict reactive species
Full Name platelet/endothelial cell adhesion molecule 1
GenBank Accession NumberBC008519
Gene Symbol CD31
Gene ID (NCBI) 18613
RRIDAB_2882960
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ08481
Storage Buffer PBS with 0.1% sodium azide and a stabilizer, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for APC CD31 antibody APC-65058Download protocol
FC protocol for APC CD31 antibody APC-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomaterials

Synergetic regulation of kupffer cells, extracellular matrix and hepatic stellate cells with versatile CXCR4-inhibiting nanocomplex for magnified therapy in liver fibrosis.

Authors - Pengkai Wu
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