CoraLite®594 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

390

Cat no : CL594-65058

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Validation Data Gallery

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  IF Staining of bEnd.3 using CL594-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite®594 CD31 antibody (CL594-65058, Clone: 390 ) at dilution of 1:200.

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  FC experiment of mouse splenocytes using CL594-65058

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD31 (CL594-65058, Clone: 390) (red), or 0.5 ug isotype control.

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Tested Applications

• Positive IF detected in: bEnd.3 cells
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65058 targets CD31 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: Pecam1
• Gene ID (NCBI): 18613
• RRID: AB_2919928
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).