Anti-Human CD314 (1D11)

CD314 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

1D11

Cat no : 65188-1-Ig

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65188-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314 (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were then stained with CL488-conjugated CD8 (CL488-65135). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65188-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314 (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65188-1-Ig targets CD314 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NKL cell line
• Full Name: killer cell lectin-like receptor subfamily K, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC039836
• Gene Symbol: KLRK1
• Gene ID (NCBI): 22914
• RRID: AB_2918459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.