CD33 Monoclonal antibody

CD33 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1B2E5

Cat no : 67135-1-Ig

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3



Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67135-1-Ig targets CD33 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD33 fusion protein Ag11402
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC028152
Gene symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD33, aslo referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 expression is restrict to cells of the hematopoietic system. It is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. This molecular may mediate cell-to-cell adhesion and act as receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Protocols

Product Specific Protocols
WB protocol for CD33 antibody 67135-1-IgDownload protocol
IHC protocol for CD33 antibody 67135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols