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CD33 Recombinant monoclonal antibody, PBS Only (84341-6-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 84341-6-RR (same clone as 84341-6-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84341-6-RR (CD33 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 84341-6-RR (same clone as 84341-6-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-6-RR (CD33 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 84341-6-RR (same clone as 84341-6-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-6-RR (CD33 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.
IHC staining of human placenta using 84341-6-RR (same clone as 84341-6-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84341-6-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.
IHC staining of human lung using 84341-6-RR (same clone as 84341-6-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 84341-6-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.
IF Staining of THP-1 using 84341-6-RR (same clone as 84341-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (84341-6-RR, Clone: 241711B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84341-6-PBS in a different storage buffer formulation.

CD33 Recombinant monoclonal antibody, PBS Only

Name: CD33 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 84341-6-PBS

CD33 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84341-6-PBS

Clone No.241711B2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3, SIGLEC 3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

84341-6-PBS targets CD33 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2138 Product name: Recombinant Human CD33 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 18-259 aa of BC028152 Sequence: DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH Predict reactive species
Full Name	CD33 molecule
Calculated Molecular Weight	364 aa, 40 kDa
Observed Molecular Weight	55-75 kDa
GenBank Accession Number	BC028152
Gene Symbol	CD33
Gene ID (NCBI)	945
RRID	AB_3743547
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P20138
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.