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Anti-Human CD33 Rabbit Recombinant Antibody, PBS Only (98184-1-PBS)

Anti-Human CD33 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD33 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98184-1-PBS-1MG

CD33 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98184-1-PBS

Clone No. 241711C12

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

241711C12, CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllFC

FC experiment of human PBMCs using 98184-1-RR (same clone as 98184-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98184-1-RR (same clone as 98184-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98184-1-PBS targets CD33 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2138 Product name: Recombinant Human CD33 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 18-259 aa of BC028152 Sequence: DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH Predict reactive species
Full Name	CD33 molecule
Calculated Molecular Weight	364 aa, 40 kDa
GenBank Accession Number	BC028152
Gene Symbol	CD33
Gene ID (NCBI)	945
RRID	AB_3672325
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P20138
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.