Tested Applications
| Positive IHC detected in | mouse spleen tissue, mouse kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31120-1-AP targets CD34 in IHC, IF, ELISA applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | CD34 antigen |
| Calculated Molecular Weight | 41 kDa |
| GenBank Accession Number | NM_133654 |
| Gene Symbol | CD34 |
| Gene ID (NCBI) | 12490 |
| RRID | AB_3669863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q64314 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD34 antibody 31120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD34 Polyclonal antibody (Cat# 31120-1-AP) has 6 total citations appearing in Bioact Mater, ACS Nano, J Am Chem Soc, ACS Appl Mater Interfaces, Sci Rep, and J Virol. Research fields include wound healing, acute kidney injury, hepatic/pulmonary fibrosis, peripheral nerve repair, diabetic cardiac ischaemia-reperfusion injury, and hantavirus-host interactions.
| Species | Application | Title |
|---|---|---|
Bioact Mater Engineering a halloysite nanotube-enhanced hydrogel 3D skin model for modulated inflammation and accelerated wound healing | ||
ACS Nano A Blood-Triggered Adhesive Hydrogel Loaded with Reactive Oxygen Species-Responsive Liposomes for the Treatment of Acute Kidney Injury. | ||
J Am Chem Soc FAP-Synergistic Organ-Targeted mRNA-LNP for Overcoming Delivery Barriers in Hepatic and Pulmonary Fibrosis. | ||
ACS Appl Mater Interfaces Suture-Free Piezoelectric Band-Aid Membrane for Complex Peripheral Nerve Defects. | ||
Sci Rep AGGF1-primed endothelial progenitor cells alleviate ischaemia-reperfusion injury in diabetic hearts. | ||
J Virol Maintenance of Hokkaido virus, a genotype of Orthohantavirus puumalaense, in the rodent host Myodes rufocanus bedfordiae under natural conditions. |
