LE/AF Purified Rabbit Anti-Human CD34 Antibody

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• LE/AF Purified Rabbit Anti-Human CD34 Antibody (BK00003)

Low-endotoxin/Azide-free (LE/AF) Purified CD34 Recombinant Antibody for IHC, FC

Cat No. BK00003

Clone No.242069A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

CD34 molecule

• 

  SEC-HPLC of BK00003

  The purity of Human CD34 Recombinant monoclonal antibody (Cat. No. BK00003) was greater than 95% as determined by SEC-HPLC.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  SDS-PAGE of BK00003

  Purity of Anti-Human CD34 Recombinant Antibody, Functional Grade (BK00003) was determined by SDS-PAGE. The antibody was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using BK00003

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using BK00003 (CD34 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using BK00003

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using BK00003 (CD34 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected HEK-293T using BK00003

  1x10^6 HEK-293T cells (left) or CD34-transfectedHEK-293Tcells (right) were surface stained with 0.25 ug LE/AF Purified Rabbit Anti-Human CD34 Antibody (BK00003, Clone: 242069A6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected HEK-293T using BK00003

  1x10^6 HEK-293T cells (left) or CD34-transfectedHEK-293Tcells (right) were surface stained with 0.25 ug LE/AF Purified Rabbit Anti-Human CD34 Antibody (BK00003, Clone: 242069A6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of BK00003

  Biolayer interferometry (BLI) kinetic assays of BK00003 against Human CD34 were performed. The affinity constant is 17.3 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Transfected CHO cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

BK00003 targets CD34 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• Conjugate: Unconjugated
• Source: Purified from CHO cell supernatant in an animal-free facility
• Form: Liquid
• Purification Method: Protein A purification
• Purity: ≥98% (HPLC), ≥98% (SDS-PAGE)
• Sterility: 0.22 µm filtration
• UNIPROT ID: P28906
• Storage Buffer: Sterile PBS, pH 7.3.
• Storage Conditions: For long-term storage, store at -80°C. Avoid repeated freeze-thaw cycles. For short-term use, store at 2-8°C for up to 2 weeks.
• Endotoxin: <0.05 EU/mg

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.