FITC Anti-Human CD34 (QBEnd-10)

• 

  FC experiment of human peripheral blood leukocyte using FITC-65183

  100 ul human peripheral blood were surface stained with 20.00 ul FITC Anti-Human CD34 (FITC-65183, Clone: QBEnd-10) and then treated with red blood cell lysis buffer. Cells were not fixed.

CD34 Monoclonal Antibody for FC

Cat No. FITC-65183

Clone No.QBEnd-10

Publications(5)

Host / Isotype

Mouse / IgG1

Reactivity

human, non-human primates

Applications

FC

Synonyms

CD34 molecule

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Tested Applications

• Positive FC detected in: human peripheral blood leukocyte

Recommended dilution

• Flow Cytometry (FC): FC : 20 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

FITC-65183 targets CD34 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human endothelial vesicles
• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• RRID: AB_2883818
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P28906
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	Front Bioeng Biotechnol; Self-assembled microtissues loaded with osteogenic MSCs for in vivo bone regeneration; Authors - Hui Li
human	FC	Cells; Exosomes from Human Omental Adipose-Derived Mesenchymal Stem Cells Secreted into Ascites Promote Peritoneal Metastasis of Epithelial Ovarian Cancer; Authors - Qingxi Qu
		Front Pharmacol; CNST is Characteristic of Leukemia Stem Cells and is Associated With Poor Prognosis in AML.; Authors - Haoyu Liu
		Mol Med Rep; Ghrelin promotes cardiomyocyte differentiation of adipose tissue‑derived mesenchymal stem cells by DDX17‑mediated regulation of the SFRP4/Wnt/β‑catenin axis; Authors - Gui-Bo Liu
human	FC	J Bioenerg Biomembr; LNX2 involves in the role of ghrelin to promote the neuronal differentiation of adipose tissue-derived mesenchymal stem cells; Authors - Gui-Bo Liu