CD37 Polyclonal antibody

CD37 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21044-1-AP

Synonyms

TSPAN26, Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40

---

Validation Data Gallery

• 

  WB analysis of rat spleen using 21044-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 21044-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21044-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21044-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 21044-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 21044-1-AP (CD37 Antibody) at dilution of 1:50.

---

Tested Applications

• Positive WB detected in: rat spleen tissue, HeLa cells, human spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

21044-1-AP targets CD37 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD37 fusion protein Ag15384
• Full Name: CD37 molecule
• Calculated Molecular Weight: 281 aa, 32 kDa
• Observed Molecular Weight: 40-52 kDa
• GenBank Accession Number: BC110379
• Gene Symbol: CD37
• Gene ID (NCBI): 951
• RRID: AB_2878800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

Publications

Species	Application	Title
human	IHC,IF	iScience; The clinical features, prognostic significance, and immune heterogeneity of CD37 in diffuse gliomas.; Authors - Xuejun Yan
mouse	IHC	Sci Rep; 89Zr-PET imaging to predict tumor uptake of 177Lu-NNV003 anti-CD37 radioimmunotherapy in mouse models of B cell lymphoma.; Authors - Danique Giesen

Related Products

CD63 Polyclonal antibody

25682-1-AP

CD9 Polyclonal antibody

20597-1-AP

CD9 Monoclonal antibody

60232-1-Ig

CD151 Polyclonal antibody

10418-1-AP