CD3 Delta Polyclonal antibody

CD3 Delta Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16669-1-AP

Synonyms

CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ

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Validation Data Gallery

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  WB analysis using 16669-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16669-1-AP

  IP Result of anti-CD3 delta (IP:16669-1-AP, 4ug; Detection:16669-1-AP 1:800) with Jurkat cells lysate 2800ug.

• 

  IF Staining of human tonsillitis using 16669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 Delta antibody (16669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 16669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 Delta antibody (16669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 16669-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD3 delta antibody (16669-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: Jurkat cells
• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16669-1-AP targets CD3 Delta in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD3 Delta fusion protein Ag10148
• Full Name: CD3d molecule, delta (CD3-TCR complex)
• Calculated molecular weight: 171 aa, 19 kDa
• Observed molecular weight: 20-25 kDa
• GenBank accession number: BC070321
• Gene symbol: CD3D
• Gene ID (NCBI): 915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publications

Species	Application	Title
mouse	IHC	Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
		Clin Cancer Res; Changes in Expression of Genes Representing Key Biologic Processes after Neoadjuvant Chemotherapy in Breast Cancer, and Prognostic Implications in Residual Disease.; Authors - Marie Klintman
human	IHC	Ann Diagn Pathol; Diagnostic utility of the aberrant immunohistochemical expression of CD3 molecules for peripheral T-cell lymphomas.; Authors - Tsugumi Satoh
mouse	WB,IP	Cell Rep; cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T cells; Authors - Yuji Fukushima
mouse	IF	Cell Discov; Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure; Authors - Jie Yin

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