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CD3 Delta Monoclonal antibody

CD3 Delta Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A12H2

Cat no : 60194-1-Ig

Synonyms

CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D

Validation Data Gallery

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  • Western Blot (WB) analysis of jurkat cells using CD3 Delta Monoclonal antibody (60194-1-Ig)

    WB analysis of jurkat using 60194-1-Ig

    jurkat cells were subjected to SDS PAGE followed by western blot with 60194-1-Ig (CD3 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected injurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60194-1-Ig is CD3 Delta Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD3 Delta fusion protein Ag10207
Full Name CD3d molecule, delta (CD3-TCR complex)
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC070321
Gene Symbol CD3 Delta
Gene ID (NCBI) 915
RRIDAB_10898175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protocols

Product Specific Protocols
WB protocol for CD3 Delta antibody 60194-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols