CD3 Gamma Polyclonal antibody

CD3 Gamma Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21120-1-AP


CD3 gamma, CD3G, CD3-gamma, T cell receptor T3 gamma chain, T3G

Tested Applications

Positive WB detected inJurkat cells, mouse thymus tissue, MOLT-4 cells
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

21120-1-AP targets CD3 Gamma in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3 Gamma fusion protein Ag15475
Full Name CD3g molecule, gamma (CD3-TCR complex)
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC113830
Gene Symbol CD3 Gamma
Gene ID (NCBI) 917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.


Product Specific Protocols
WB protocol for CD3 Gamma antibody 21120-1-APDownload protocol
IHC protocol for CD3 Gamma antibody 21120-1-APDownload protocol
IP protocol for CD3 Gamma antibody 21120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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