CD4 Polyclonal antibody

• 

  WB analysis of human spleen using 11056-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11056-2-AP (CD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen tissue using 11056-2-AP

  IP result of anti-CD4 (11056-2-AP for IP and Detection) with mouse spleen tissue lysate.

CD4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 11056-2-AP

Publications(19)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ELISA

Synonyms

CD4, CD4 molecule, CD4mut

---

Tested Applications

• Positive WB detected in: human spleen tissue
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

11056-2-AP targets CD4 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0422

  Product name: Recombinant human CD4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: CD4 of BC025782

  Sequence:

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• RRID: AB_2073067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01730
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		ACS Appl Mater Interfaces; NIR-Triggered Sequentially Responsive Nanocarriers Amplified Cascade Synergistic Effect of Chemo-Photodynamic Therapy with Inspired Antitumor Immunity.; Authors - Feiyang Jin
human		Cancer Lett; A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.; Authors - Huimin Tao
human	WB	Front Immunol; PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation; Authors - Rui Su
mouse		EBioMedicine; Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.; Authors - Feng Gao
		Am J Cancer Res; Changes in the intestinal microecology induced by bacillus subtilis inhibit the occurrence of ulcerative colitis and associated cancers: a study on the mechanisms.; Authors - Chuancong Wu
mouse		J Transl Med; Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.; Authors - Han Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Luca (Verified Customer) (02-26-2018)

• Applications: Other (please state below),
• Primary Antibody Dilution: 10000 to 1 ng/ml