CD4 Polyclonal antibody

CD4 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Cat no : 11056-2-AP

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

11056-2-AP targets CD4 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD4 fusion protein Ag0422
Full Name CD4 molecule
Calculated molecular weight 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Protocols

Product Specific Protocols
WB protocol for CD4 antibody 11056-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

ACS Appl Mater Interfaces

NIR-Triggered Sequentially Responsive Nanocarriers Amplified Cascade Synergistic Effect of Chemo-Photodynamic Therapy with Inspired Antitumor Immunity.

Authors - Feiyang Jin
humanIHC

Cancer Lett

A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.

Authors - Huimin Tao
mouseIF

EBioMedicine

Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.

Authors - Feng Gao

Am J Cancer Res

Changes in the intestinal microecology induced by bacillus subtilis inhibit the occurrence of ulcerative colitis and associated cancers: a study on the mechanisms.

Authors - Chuancong Wu
mouse IF

J Transl Med

Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.

Authors - Han Wang
humanIHC

Cancer Sci

Sonodynamic therapy-assisted immunotherapy: A novel modality for cancer treatment.

Authors - Qianyu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Luca (Verified Customer) (02-26-2018)

  • Applications: Other (please state below),
  • Primary Antibody Dilution: 10000 to 1 ng/ml