|Positive WB detected in||human spleen tissue|
|Positive IP detected in||mouse spleen tissue|
|Western Blot (WB)||WB : 1:500-1:2400|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
11056-2-AP targets CD4 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD4 fusion protein Ag0422|
|Full Name||CD4 molecule|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC025782|
|Gene ID (NCBI)||920|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).
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Luca (Verified Customer) (02-26-2018)