CD4 Polyclonal antibody

CD4 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19068-1-AP

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive WB detected inmouse thymus tissue
Positive IP detected inmouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

19068-1-AP targets CD4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD4 fusion protein Ag13616
Full Name CD4 molecule
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Protocols

Product Specific Protocols
WB protocol for CD4 antibody 19068-1-APDownload protocol
IP protocol for CD4 antibody 19068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Control Release

Cancer-cell-biomimetic Upconversion nanoparticles combining chemo-photodynamic therapy and CD73 blockade for metastatic triple-negative breast cancer.

Authors - Feiyang Jin
humanIHC

J Hematol Oncol

PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse.

Authors - Bingxin Zheng
ratIHC

Exp Neurol

Calpain mediated expansion of CD4+ cytotoxic T cells in rodent models of Parkinson's disease.

Authors - Azizul Haque
mouseIF

Food Funct

Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.

Authors - Jinhong Xu
humanIHC

Mol Med

PRSS1 mutation: a possible pathomechanism of pancreatic carcinogenesis and pancreatic cancer.

Authors - Qicai Liu
mouseWB

CNS Neurosci Ther

Naltrexone is neuroprotective against traumatic brain injury in mu opioid receptor knockout mice.

Authors - Yu-Syuan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nicole (Verified Customer) (04-28-2022)

no signal expression is controlled by other antibodies

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela, Plasmid driven expression of various CD4 fusion constructs