CD4 Polyclonal antibody

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  WB analysis using 32406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32406-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat thymus using 32406-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 32406-1-AP (CD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thymus using 32406-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 32406-1-AP (CD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat spleen using 32406-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD4 antibody (32406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat spleen using 32406-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD4 antibody (32406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 32406-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD4 molecule, p55

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Tested Applications

• Positive WB detected in: rat spleen tissue, rat thymus tissue
• Positive IHC detected in: rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32406-1-AP targets CD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg2025

  Product name: Recombinant Rat CD4 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 28-394 aa of NM_012705.1

  Sequence: KTVVLGKEGGSAELPCESTSRRSASFAWKSSDQKTILGYKNKLLIKGSLELYSRFDSRKNAWERGSFPLIINKLRMEDSQTYVCELENKKEEVELWVFRVTFNPGTRLLQGQSLTLILDSNPKVSDPPIECKHKSSNIVKDSKAFSTHSLRIQDSGIWNCTVTLNQKKHSFDMKLSVLGFASTSITAYKSEGESAEFSFPLNLGEESLQGELRWKAEKAPSSQSWITFSLKNQKVSVQKSTSNPKFQLSETLPLTLQIPQVSLQFAGSGNLTLTLDRGILYQEVNLVVMKVTQPDSNTLTCEVMGPTSPKMRLILKQENQEARVSRQEKVIQVQAPEAGVWQCLLSEGEEVKMDSKIQVLSKGLNQT

• Full Name: Cd4 molecule
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: NM_012705.1
• Gene Symbol: Cd4
• Gene ID (NCBI): 24932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P05540
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).