Anti-Human CD4 (RPA-T4)

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  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

CD4 Monoclonal Antibody for FC

Cat No. 65143-1-Ig

Clone No.RPA-T4

Publications(1)

Reviews (3)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF, FC

Synonyms

CD4 molecule, CD4mut, RPA-T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65143-1-Ig targets CD4 in IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• RRID: AB_2918429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01730
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Allergy Asthma Immunol Res; TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis.; Authors - Lu Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sunil (Verified Customer) (08-03-2023)

worked as expected for conjugating our custom dyes to

• Applications: Flow Cytometry

FH

Sareena (Verified Customer) (06-02-2023)

Worked well with compensation beads

• Applications: Immunofluorescence, Flow Cytometry

FH

Sareena (Verified Customer) (04-20-2023)

Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 100 ug
• Cell Tissue Type: CD4 RPA-T4 antibody