Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65143-1-Ig targets CD4 in IF, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD4 molecule |
| Calculated Molecular Weight | 51 kDa |
| GenBank Accession Number | BC025782 |
| Gene Symbol | CD4 |
| Gene ID (NCBI) | 920 |
| ENSEMBL Gene ID | ENSG00000010610 |
| RRID | AB_2918429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01730 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD4 antibody 65143-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD4 (RPA-T4) antibody (Cat# 65143-1-Ig) has 3 citations published in Materials Today Bio, Allergy Asthma Immunology Research, and Molecular Carcinogenesis. The associated research fields cover prostate cancer nanomedicine therapy, allergic rhinitis immunology involving T-cell balance, and melanoma resistance to anti-PD-1 immunotherapy. Cited applications include flow cytometry and immunofluorescence, with species spanning human and mouse.
| Species | Application | Title |
|---|---|---|
Mater Today Bio 3-acetyl-11-keto-β-boswellic acid and chitosan-Ag nanoparticles for synergistic tumor-resident bacteria mediated prostate cancer therapy. | ||
Allergy Asthma Immunol Res TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis. | ||
Mol Carcinog POU4F1 Promotes the Primary Resistance of Melanoma to Anti-PD-1 Therapy by Regulating Glycolysis Through METTL1-Mediated m7G Methylation of PKM2. |
Reviews
What customers say
All three reviewers gave this product a perfect 5-star rating. Users consistently reported that it performed as expected for conjugating custom dyes and fluorophores to antibodies. Multiple reviewers highlighted its compatibility with compensation beads for flow cytometry analysis. One user specifically noted successful use with CD4 RPA-T4 antibody at a 100 µg scale. Overall, customers found it reliable and effective for flow cytometry and immunofluorescence applications, with no negative feedback reported across any lot or application tested.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sunil (Verified Customer) (08-03-2023) | worked as expected for conjugating our custom dyes to
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Sareena (Verified Customer) (06-02-2023) | Worked well with compensation beads
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Sareena (Verified Customer) (04-20-2023) | Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.
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