CD4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83513-7-RR (same clone as 83513-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83513-7-RR (CD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 83513-7-RR (same clone as 83513-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD4 antibody (83513-7-RR, Clone: 240427E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83513-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83513-7-RR against Human CD4 were performed. The affinity constant is 0.426 nM.

CD4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83513-7-PBS

Clone No.240427E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Product Information

83513-7-PBS targets CD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0333

  Product name: Recombinant Human CD4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-396 aa of BC025782

  Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.