Anti-Rat CD4 Rabbit Recombinant Antibody

• 

  FC experiment of rat splenocytes using 98252-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD4 Rabbit Recombinant Antibody (98252-1-RR, Clone: 241955C11) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of rat splenocytes using 98252-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD4 Rabbit Recombinant Antibody (98252-1-RR, Clone: 241955C11) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD4 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98252-1-RR

Clone No.241955C11

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

241955C11, CD4 molecule, p55

---

Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98252-1-RR targets CD4 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD4 fusion protein Eg2025
• Full Name: Cd4 molecule
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: NM_012705.1
• Gene Symbol: Cd4
• Gene ID (NCBI): 24932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05540
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).