Anti-Mouse CD4 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98522-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98522-3-RR against Mouse CD4 were performed. The affinity constant is below 1 pM.

CD4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98522-3-PBS

Clone No.250263B7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

---

Product Information

98522-3-PBS targets CD4 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3847

  Product name: Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 27-394 aa of NM_013488

  Sequence: KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

• Full Name: CD4 antigen
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: NM_013488
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06332
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).