Anti-Guinea Pig CD4 Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• Anti-Guinea Pig CD4 Rabbit Recombinant Antibody (98642-3-RR)

• 

  FC experiment of guinea pig splenocytes using 98642-3-RR

  1x10^6 guinea pig splenocytes cells were surface stained with 0.25 ug Anti-Guinea Pig CD4 Rabbit RecAb (98642-3-RR, Clone:251660F2),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD11b. Cells were not fixed.

• 

  FC experiment of guinea pig splenocytes using 98642-3-RR

  1x10^6 guinea pig splenocytes cells were surface stained with 0.25 ug Anti-Guinea Pig CD4 Rabbit RecAb (98642-3-RR, Clone:251660F2)(red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue). Cells were not fixed.

• 

  Affinity of 98642-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98642-3-RR against Guinea Pig CD4 were performed. The affinity constant is below 1 pM.

CD4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98642-3-RR

Clone No.251660F2

Host / Isotype

Rabbit / IgG

Reactivity

guinea pig

Applications

FC

Synonyms

CD4 molecule

---

Tested Applications

• Positive FC detected in: guinea pig splenocytes cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98642-3-RR targets CD4 in FC applications and shows reactivity with guinea pig samples.

• Tested Reactivity: guinea pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg7150

  Product name: Recombinant guinea pig CD4 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 38-408 aa of XM_013145124.1

  Sequence: KDVVLSKEGATVELPCIGSQRKNMPFSWKFLNETKILAKQNSYLTRGKTHLGNRIDSRSSQWDRGSFPLIIRKLEMKDSEIYICEVDNKRMLVELKVFKLTASPGTRLVEGQTLNLIVEGPKNSQLPVQFTSPAGQIFKEEAAITVPSVGFQHNGIWRCIVTLDKKYLQLDIYILVLSFQKMQIPNPFYKKEKESLEMCFPLTFNNEDLPFTGELIWRADKASSSHSWITFSVANQRVSVQNDNQDPKLQMAEKTPLCLTLSSVLLQHAGSGNLTLTLDGGQRLSQEVKLVVMKVAQDQNSLVCEVQGPTSSTLMLSWNLKDQKPQVSKQEKQVWVLSPEAGTWQCLLSDGSQVLLESTVDVVSRGMPQDQ

• Full Name: CD4 molecule
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: XM_013145124.1
• Gene Symbol: Cd4
• Gene ID (NCBI): 100734494
• RRID: AB_3746239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: H0VL66
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).