APC Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

RM4-5

Cat no : APC-65141

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC Anti-Mouse CD4 (APC-65141, Clone: RM4-5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC-Rat IgG2a isotype control antibody. Cells were not fixed.

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  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug APC-anti-mouse CD4 (APC-65141, clone RM4-5) (red) or 0.20 ug APC-rat IgG2a isotype control (blue). Cells were not fixed. .

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65141 targets CD4 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: BALB/c mouse thymocytes
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• RRID: AB_2883022
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).