CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65141
Clone No.RM4-5

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC

Cd4, CD4 antigen, L3T4, Ly 4

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

CL488-65141 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen BALB/c mouse thymocytes Predict reactive species
Full Name CD4 antigen
GenBank Accession NumberBC039137
Gene Symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883201
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
FC protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nat Commun

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis

Authors - Xinyan Zhou
mouseIF

Cell Commun Signal

Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma

Authors - Yuqi Yang
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