CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

RM4-5

Cat no : CL488-65141

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL488-65141

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL488-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL488-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated Rat IgG2a isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:250-1:1000

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

CL488-65141 targets CD4 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: BALB/c mouse thymocytes
• Full Name: CD4 antigen
• GenBank accession number: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
	IF	Nat Commun; In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis; Authors - Xinyan Zhou