PE Anti-Rat CD4 (OX-35)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2a kappa

Reactivity

Rat

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX-35

Cat no : PE-65179

Synonyms

CD4, CD4 molecule, p55, W3/25

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Validation Data Gallery

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  FC experiment of wistar rat splenocytes using PE-65179

  1X10^6 Wistar rat splenocytes were surface stained with 0.25 ug PE Anti-Rat CD4 (PE-65179, Clone: OX-35) (red) or 0.25 ug isotype control antibody (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65179 targets CD4 in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / Mouse IgG2a kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLR generated rat T helper lymphocytes
• Full Name: Cd4 molecule
• Gene Symbol: Cd4
• Gene ID (NCBI): 24932
• RRID: AB_2883951
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).