Anti-Human CD41 (HIP8)

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  FC experiment of human peripheral blood using 65173-1-Ig

  100 ul human platelet rich plasma (diluted 1:4 with PBS) was surface stained with 0.2 ug Anti-Human CD41a (65173-1-Ig, Clone:HIP8) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

• 

  FC experiment of human peripheral blood using 65173-1-Ig

  100 ul human platelet rich plasma (diluted 1:4 with PBS) was surface stained with 0.2 ug Anti-Human CD41a (65173-1-Ig, Clone:HIP8) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

CD41 Monoclonal Antibody for FC

Cat No. 65173-1-Ig

Clone No.HIP8

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Synonyms

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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Tested Applications

• Positive FC detected in: human peripheral blood

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65173-1-Ig targets CD41 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• GenBank Accession Number: BC117443
• Gene Symbol: CD41a
• Gene ID (NCBI): 3674
• RRID: AB_2881482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08514
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.