CoraLite® Plus 647 Anti-Human CD41 (HIP8)

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  FC experiment of human peripheral blood platelets using CL647-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD41a (CL647-65173, Clone:HIP8) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using CL647-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD41a (CL647-65173, Clone:HIP8) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124). Cells were not fixed.

CD41 Monoclonal Antibody for FC

Cat No. CL647-65173

Clone No.HIP8

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Synonyms

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65173 targets CD41 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• GenBank Accession Number: BC117443
• Gene Symbol: CD41a
• Gene ID (NCBI): 3674
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Unknow
• UNIPROT ID: P08514
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.