CD42b Monoclonal antibody, PBS Only

CD42b Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G1F5

Cat no : 67857-1-PBS

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 67857-1-Ig (same clone as 67857-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 67857-1-Ig (same clone as 67857-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67857-1-PBS targets CD42b in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD42b fusion protein Ag31181
• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 626 aa, 69 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC027955
• Gene Symbol: GP1BA
• Gene ID (NCBI): 2811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.