Filter: AllWBIHC

WB analysis of human peripheral blood platelets using 67857-1-Ig (same clone as 67857-1-PBS)
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.
WB analysis of human blood using 67857-1-Ig (same clone as 67857-1-PBS)
human blood were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67857-1-Ig (same clone as 67857-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

CD42b Monoclonal antibody, PBS Only

CD42b Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67857-1-PBS

Clone No.3G1F5

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67857-1-PBS targets CD42b in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag31181 Product name: Recombinant human GP1BA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-350 aa of BC027955 Sequence: ICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTK Predict reactive species
Full Name	glycoprotein Ib (platelet), alpha polypeptide
Calculated Molecular Weight	626 aa, 69 kDa
Observed Molecular Weight	145 kDa
GenBank Accession Number	BC027955
Gene Symbol	CD42b
Gene ID (NCBI)	2811
ENSEMBL Gene ID	ENSG00000185245
RRID	AB_2918615
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P07359
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.