CD42b Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of human peripheral blood platelets using 87285-3-RR (same clone as 87285-3-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 87285-3-RR (CD42b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87285-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HEL using 87285-3-RR (same clone as 87285-3-PBS)

  HEL cells were subjected to SDS PAGE followed by western blot with 87285-3-RR (CD42b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87285-3-PBS in a different storage buffer formulation.

CD42b Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87285-3-PBS

Clone No.252200G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Antigen CD42b-alpha, BSS, CD42b alpha, GP1B, GP1BA

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Product Information

87285-3-PBS targets CD42b in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3051

  Product name: Recombinant human GP1BA protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-505 aa of NM_000173.7

  Sequence: HPICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTKEQTTFPPRWTPNFTLHMESITFSKTPKSTTEPTPSPTTSEPVPEPAPNMTTLEPTPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTPIPTIATSPTILVSATSLITPKSTFLTTTKPVSLLESTKKTIPELDQPPKLR

• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: NM_000173.7
• Gene Symbol: CD42b
• Gene ID (NCBI): 2811
• ENSEMBL Gene ID: ENSG00000185245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07359
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).