Anti-Human CD42b Rabbit Recombinant Antibody

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• Anti-Human CD42b Rabbit Recombinant Antibody (98752-3-RR)

CD42b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98752-3-RR

Clone No.252200B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Antigen CD42b-alpha, BSS, CD42b alpha, GP1B, GP1BA

• 

  FC experiment of human peripheral blood platelets using 98752-3-RR

  1x10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD42b Rabbit RecAb (98752-3-RR, Clone: 252200B12) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 98752-3-RR

  Biolayer interferometry (BLI) kinetic assays of 98752-3-RR against Human CD42b were performed. The affinity constant is 2.80 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98752-3-RR targets CD42b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3051

  Product name: Recombinant human GP1BA protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-505 aa of NM_000173.7

  Sequence: HPICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTKEQTTFPPRWTPNFTLHMESITFSKTPKSTTEPTPSPTTSEPVPEPAPNMTTLEPTPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTPIPTIATSPTILVSATSLITPKSTFLTTTKPVSLLESTKKTIPELDQPPKLR

• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 72 kDa
• GenBank Accession Number: NM_000173.7
• Gene Symbol: CD42b
• Gene ID (NCBI): 2811
• ENSEMBL Gene ID: ENSG00000185245
• RRID: AB_3746320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07359
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).