CD42c Polyclonal antibody

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  WB analysis using 55161-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 55161-1-AP (CD42c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CD42c Polyclonal Antibody for WB, ELISA

Cat No. 55161-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GP1BB, GPIbB, GPIb beta, GP Ib beta, Antigen CD42b beta

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55161-1-AP targets CD42c in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycoprotein Ib (platelet), beta polypeptide
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM_000407
• Gene Symbol: CD42c
• Gene ID (NCBI): 2812
• RRID: AB_2881279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13224
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD42c, also known as platelet glycoprotein Ib beta chain (GP1BB), is a transmembrane protein containing a leucine-rich amino acid sequence (PMID: 3353370). CD42c is covalently linked to platelet glycoprotein Ib alpha chain (CD42b/GP1BA) to form GPIb, which is part of the Glycoprotein Ib-V-IX receptor (GPIb-V-IX) complex that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion (PMID: 7660135). Mutations in the GP1BB gene have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome, and giant platelet disorder.