CD44 Polyclonal antibody

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  WB analysis using 31981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31981-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat spleen using 31981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD44 antibody (31981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat spleen using 31981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD44 antibody (31981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD44 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 31981-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat lung tissue, rat spleen tissue
• Positive IF-P detected in: rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31981-1-AP targets CD44 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg1458

  Product name: Recombinant Rat CD44 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-271 aa of XM_006234631.3

  Sequence: QQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQMELALSKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGDRDDAFFIGSTLATSDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPE

• Full Name: Cd44 molecule
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: XM_006234631.3
• Gene Symbol: Cd44
• Gene ID (NCBI): 25406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O70509
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.