CD45 Recombinant monoclonal antibody, PBS Only

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  IHC staining of human tonsillitis using 84405-3-RR (same clone as 84405-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-3-RR (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84405-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84405-3-RR (same clone as 84405-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-3-RR (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84405-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 84405-3-RR (same clone as 84405-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using CD45 antibody (84405-3-RR, Clone: 241671A10 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84405-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84405-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84405-3-RR against Human CD45 were performed. The affinity constant is 3.66 nM.

CD45 Uni-rAb^® Recombinant Antibody for IHC, IF-Fro, Indirect ELISA

Cat No. 84405-3-PBS

Clone No.241671A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-Fro, Indirect ELISA

Synonyms

241671A10, EC:3.1.3.48, GP180, L CA, LCA

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Product Information

84405-3-PBS targets CD45 in IHC, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2486

  Product name: recombinant human CD45(220-577) protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007019 of

  Sequence:

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 147 kDa
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08575
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.