FITC Anti-Human CD45 (F10-89-4 )

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

F10-89-4

Cat no : FITC-65064

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using FITC-65064

  1X10^6 human peripheral blood lymphocytes were surface stained with 10 ul FITC-Anti-Human CD45 (FITC-65064, clone F10-89-4) (red) or 10 ul FITC-mouse IgG2a isotype control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65064 targets CD45 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified T cells from human lymph nodes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank accession number: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).