Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for IHC, IF-P, FC
Cat No. 65226-1-Ig
Clone No.F8-11-13

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IHC, IF-P, FC

CD45, EC:3.1.3.48, F8-11-13, GP180, L CA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65226-1-Ig targets CD45RA in IHC, IF-P, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T lymphocytes Predict reactive species
Full Name protein tyrosine phosphatase, receptor type, C
Calculated Molecular Weight 1304 aa, 147 kDa
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3084907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08575
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
IHC protocol for CD45RA antibody 65226-1-IgDownload protocol
IF protocol for CD45RA antibody 65226-1-IgDownload protocol
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
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