• Featured Product
  • KD/KO Validated

CD46 Polyclonal antibody

CD46 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12494-1-AP

Synonyms

CD46, MCP, Membrane cofactor protein, MIC10, TLX, TRA2.10



Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12494-1-AP targets CD46 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD46 fusion protein Ag3131
Full Name CD46 molecule, complement regulatory protein
Calculated Molecular Weight 384 aa, 43 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC030594
Gene Symbol CD46
Gene ID (NCBI) 4179
RRIDAB_2260042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CD46 antibody 12494-1-APDownload protocol
IHC protocol for CD46 antibody 12494-1-APDownload protocol
IF protocol for CD46 antibody 12494-1-APDownload protocol
IP protocol for CD46 antibody 12494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, FC

Mol Immunol

Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.

Authors - Frida C Mohlin
humanWB

Oncol Rep

Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.

Authors - Zejun Lu
  • KD Validated
mouseWB

Clin Exp Immunol

Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.

Authors - Yingchun Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (04-12-2022)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa cells