Mouse CD48 Polyclonal antibody, PBS Only

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• Mouse CD48 Polyclonal antibody, PBS Only (31774-1-PBS)

• 

  WB analysis using 31774-1-AP (same clone as 31774-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31774-1-AP (CD48/SLAMF2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

CD48 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31774-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102

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Product Information

31774-1-PBS targets CD48 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg1323

  Product name: recombinant mouse CD48/SLAMF2 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 23-217 aa of BAE96326.1

  Sequence: FQGHSIPDINATTGSNVTLKIHKDPLGPYKRITWLHTKNQKILEYNYNSTKTIFESEFKGRVYLEENDGALHISNVRKEDKGTYYMRVLRETENELKITLEVFDPVPKPSIEINKTEASTDSCHLRLSCEVKDQHVDYTWYESSGPFPKKSPGYVLDLIVTPQNKSTFYTCQVSNPVSSKNDTVYFTLPCDLARS

• Full Name: CD48 antigen
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BAE96326.1
• Gene Symbol: Cd48
• Gene ID (NCBI): 12506
• RRID: AB_3670109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P18181
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.