Anti-Mouse CD48 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone: 240732A5) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone:240732A5) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98081-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98081-1-RR against Mouse CD48 were performed. The affinity constant is 1.48 nM.

CD48 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98081-1-RR

Clone No.240732A5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98081-1-RR targets CD48 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD48 antigen
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BAE96326.1
• Gene Symbol: Cd48
• Gene ID (NCBI): 12506
• RRID: AB_3672228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P18181
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.