Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

1

CloneNo.

DX5

Cat no : 65065-1-Ig

Synonyms

CD49B, DX5, integrin alpha 2, Itga2

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65065-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD49b (65065-1-Ig, Clone: DX5) and FITC anti-rat IgM Antibody at dilution 1:50 (green), or stained with 0.5 ug rat IgM isotype control and FITC anti-rat IgM Antibody at dilution 1:50 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

65065-1-Ig targets CD49b in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NK cells isolated from C57BL/6 mice
• Full Name: integrin alpha 2
• GenBank Accession Number: BC065139
• Gene Symbol: Itga2
• Gene ID (NCBI): 16398
• RRID: AB_2918372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

Publications

Species	Application	Title
mouse	FC	Nanoscale Adv; Bacteria-mediated tumor immunotherapy via photothermally-programmed PD1 expression; Authors - Wenxuan Xu