Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 1 publications below |
Product Information
APC-65065 targets CD49b in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
NK cells isolated from C57BL/6 mice Predict reactive species |
| Full Name | integrin alpha 2 |
| GenBank Accession Number | BC065139 |
| Gene Symbol | CD49b |
| Gene ID (NCBI) | 16398 |
| RRID | AB_2882967 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q62469 |
| Storage Buffer | PBS with 0.1% sodium azide and a stabilizer, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD49b antibody APC-65065 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
