APC Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

mouse

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

1

CloneNo.

DX5

Cat no : APC-65065

Synonyms

Itga2, integrin alpha 2, DX5

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC Anti-Mouse CD49b (APC-65065, Clone: DX5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 0.20 ug APC-Rat IgM isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

APC-65065 targets CD49b in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NK cells isolated from C57BL/6 mice
• Full Name: integrin alpha 2
• GenBank Accession Number: BC065139
• Gene Symbol: CD49b
• Gene ID (NCBI): 16398
• RRID: AB_2882967
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.

Publications

Species	Application	Title
	FC	Liver Int; Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury; Authors - Yuan Li