CoraLite® Plus 488 Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DX5

Cat no : CL488-65065

Synonyms

CD49B, DX5, integrin alpha 2, Itga2

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using CL488-65065

  1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (PK136) (APC-65138, Clone: PK136) and CoraLite® Plus 488-conjugated Rat IgM isotype control antibody (left) or 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD49b (CL488-65065, Clone: DX5) (right). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65065 targets CD49b in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NK cells isolated from C57BL/6 mice
• Full Name: integrin alpha 2
• GenBank Accession Number: BC065139
• Gene Symbol: CD49b
• Gene ID (NCBI): 16398
• RRID: AB_2883158
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.