PE Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

DX5

Cat no : PE-65065

Synonyms

Itga2, integrin alpha 2, DX5

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (APC-65138, Clone: PK136) and 0.20 ug PE Anti-Mouse CD49b (PE-65065, Clone: DX5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65065

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (APC-65138, Clone: PK136) and 0.20 ug PE-Rat IgM isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65065 targets CD49b in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NK cells isolated from C57BL/6 mice
• Full Name: integrin alpha 2
• GenBank Accession Number: BC065139
• Gene Symbol: CD49b
• Gene ID (NCBI): 16398
• RRID: AB_2883862
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.