Anti-Human CD52 Rabbit Recombinant Antibody

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• Anti-Human CD52 Rabbit Recombinant Antibody (98750-1-RR)

CD52 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98750-1-RR

Clone No.253542A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Cambridge pathology 1 antigen, CAMPATH 1 antigen, CAMPATH-1 antigen, CD52 molecule, He5

• 

  FC experiment of human peripheral blood leukocytes using 98750-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD52 Rabbit RecAb (98750-1-RR, Clone: 253542A1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood leukocytes using 98750-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD52 Rabbit RecAb (98750-1-RR, Clone: 253542A1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98750-1-RR targets CD52 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: CD52 molecule
• Gene Symbol: CD52
• Gene ID (NCBI): 1043
• RRID: AB_3746317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31358
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD52 (Cluster of Differentiation 52), also known as the CAMPATH-1 antigen, is a small, heavily glycosylated protein found on the surface of various immune and reproductive cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs) (PMID: 16797237). CD52 is tethered to outer surface of cellular membrane by glycosylphosphatidylinositol (GPI)-anchor, and does not possess intracellular domains (PMID: 19236377, 28283679).