APC Anti-Human CD54 (ICAM-1) (15.2)

CD54 (ICAM-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

1

CloneNo.

15.2

Cat no : APC-65075

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Validation Data Gallery

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  FC experiment of human peripheral blood monocytes using APC-65075

  1X10^6 human peripheral blood monocytes were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone: 15.2) (blue), or isotype control antibody (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood monocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

• FC: See 1 publications below

Product Information

APC-65075 targets CD54 (ICAM-1) in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: intercellular adhesion molecule 1
• Calculated molecular weight: 90 kDa
• GenBank accession number: BC015969
• Gene symbol: ICAM1
• Gene ID (NCBI): 3383
• Conjugate: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Publications

Species	Application	Title
	FC	Cell Signal; RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.; Authors - Wenjing Li