APC Anti-Human ICAM-1/CD54 (15.2)

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  FC experiment of human PBMCs using APC-65075

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) (red) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (blue). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using APC-65075

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

ICAM-1/CD54 Monoclonal Antibody for FC

Cat No. APC-65075

Clone No.15.2

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

APC-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• ENSEMBL Gene ID: ENSG00000090339
• RRID: AB_2882974
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P05362
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Publications

Species	Application	Title
		Adv Healthc Mater; An Erythrocyte Membrane-Derived Nanosystem for Efficient Reversal of Endothelial Injury in Sepsis; Authors - Yao Zhang
	FC	Cell Signal; RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.; Authors - Wenjing Li