APC Anti-Human CD54 (ICAM-1) (15.2)

CD54 (ICAM-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

15.2

Cat no : APC-65075

Synonyms

BB2, CD54, CD54 (ICAM-1), ICAM 1, ICAM1, P3.58



Tested Applications

Positive FC detected inhuman peripheral blood monocytes
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl(0.5µg) per 10^6 cells in a 100 µl suspension or 5 µl(0.5µg) per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

APC-65075 targets CD54 (ICAM-1) in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM1
Gene ID (NCBI) 3383
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for APC CD54 (ICAM-1) antibody APC-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Cell Signal

RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.

Authors - Wenjing Li