Mouse CD6 Recombinant monoclonal antibody, PBS Only

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  WB analysis of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84166-5-RR (CD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84166-5-RR (CD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84166-5-RR (CD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD6 antibody (84166-5-RR, Clone: 241471H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

CD6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84166-5-PBS

Clone No.241471H6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241471H6, T-cell differentiation antigen CD6

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Product Information

84166-5-PBS targets CD6 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1401

  Product name: Recombinant Mouse CD6 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-396 aa of NM_009852.3

  Sequence: GLPSPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCAAHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQDQECSSDRRLVWVTCAENQAVRLVDGSSRCAGRVEMLEHGEWGTVCDDTWDLQDAHVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGIDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCRSLGCGSAVARPRGLPHSLDGRMYYSCKGQEPALSTCSWRFNNSNLCSQSRAARVVCSGSQRHLNLSTSEVPSRVPVTIESSVPVSVKDKDSQG

• Full Name: CD6 antigen
• Calculated Molecular Weight: 72kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_009852.3
• Gene Symbol: Cd6
• Gene ID (NCBI): 12511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q61003-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD6 is a type I transmembrane glycoprotein of 105-130 kDa that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harbouring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.