CD6 Recombinant monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 84508-5-RR (same clone as 84508-5-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 84508-5-RR (CD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84508-5-RR (same clone as 84508-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84508-5-RR (same clone as 84508-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84508-5-RR (same clone as 84508-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD6 antibody (84508-5-RR, Clone: 241965D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

CD6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84508-5-PBS

Clone No.241965D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

241965D11, CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6

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Product Information

84508-5-PBS targets CD6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2079

  Product name: Recombinant Human CD6 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 18-398 aa of BC033755

  Sequence: HPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRE

• Full Name: CD6 molecule
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC033755
• Gene Symbol: CD6
• Gene ID (NCBI): 923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P30203
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.