Anti-Human CD61 (VIPL2)

CD61 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Non-human primates and More (1)

Applications

IF, FC

Conjugate

Unconjugated

Publications

1

CloneNo.

VIPL2

Cat no : 65185-1-Ig

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using 65185-1-Ig

  Human peripheral blood platelets were surface stained with Purified Anti-Human CD61 (65185-1-Ig, Clone:VIPL2) (red) or Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65185-1-Ig targets CD61 in IF, FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Cited Reactivity: human
• Host / Isotype: Mouse / Mouse IgG1 kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Calculated Molecular Weight: 788 aa, 87 kDa
• GenBank Accession Number: BC127666
• Gene Symbol: ITGB3
• Gene ID (NCBI): 3690
• RRID: AB_2881484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C.

Background Information

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).

Publications

Species	Application	Title
human	IF	Small Methods; Superresolution Fluorescence Microscopy of Platelet Subcellular Structures as a Potential Tumor Liquid Biopsy; Authors - Peng Xu